Dr. Amy  Gabriel  Md image

Dr. Amy Gabriel Md

707 Druid Rd E
Clearwater FL 33756
727 611-1439
Medical School: University Of Iowa College Of Medicine - 1999
Accepts Medicare: Yes
Participates In eRX: No
Participates In PQRS: No
Participates In EHR: No
License #: ME114380
NPI: 1902980717
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Energetics and mechanism for the deamination of lithiated cysteine. - The journal of physical chemistry. B
Lithium cation complexes with cysteine (Cys) are collisionally activated with xenon in a guided ion beam tandem mass spectrometer and observed to deaminate in addition to loss of the intact amino acid. Source conditions are found to influence the cross sections for these processes considerably, a result interpreted in terms of two isomers, Li(+)(Cys) and (NH(3))Li(+)(C(3)H(4)O(2)S). Quantum chemical calculations at the B3LYP/6-311G(d,p) level are used to explore the reaction mechanism for this fragmentation process in detail. A complete reaction coordinate surface for the process is elucidated, including all intermediates and transition states. Theoretical molecular parameters for the two isomers and for the rate-limiting transition state for deamination of Li(+)(Cys) are then used to analyze the threshold energies in the experimental data, providing experimental measurements of the energies of the transition state and various products. These experimental energies are compared with single point energies calculated at three different levels, B3LYP, B3P86, and MP2(full), using the 6-311+G(2d,2p) basis set with geometries and zero point energies calculated at the B3LYP/6-311G(d,p) level, as well as with additional calculations using basis sets that include core correlation on lithium. Good agreement between experiment and theory suggests that the reaction mechanisms have been reasonably elucidated and identifies the C(3)H(4)O(2)S deamination product as thiirane-carboxylic acid.

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