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NIR fluorescence studies of Neodymium ions doped sodium fluoroborate glasses for 1.06Î¼m laser applications. - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
The concentration dependent luminescence properties of Nd(3+) ions doped lead calcium zinc sodium fluoroborate (LCZSFB) glasses were prepared via a melt quenching technique and reported through optical absorption, NIR luminescence and lifetime measurements. The optical transition properties of Nd(3+) ions have been analyzed using Judd-Ofelt theory. The present glasses have high stimulated emission cross - sections, entail that they have high potential laser applications. The decay curves of all the glasses show single exponential behavior. The discrepancy between the experimental and calculated lifetimes of emitting level was ascribed to energy transfer through cross-relaxation. The estimated values of radiative and saturation intensity of (4)F3/2â†’(4)I11/2 transition indicates the favorable lasing action at 1.06Î¼m.Copyright Â© 2013 Elsevier B.V. All rights reserved.
Protective effect of a Butea monosperma (Lam.) Taub. flowers extract against skin inflammation: antioxidant, anti-inflammatory and matrix metalloproteinases inhibitory activities. - Journal of ethnopharmacology
Butea monosperma (Lam.) Taubert (Syn. Butea frondosa; family Fabaceae) is a common plant of the Indian continent (Das et al., 2011; Sharma and Deshwal, 2011). The brightly orange flowers of this plant are widely used in traditional medicine and more particularly for inflammatory disease.In vitro anti-inflammatory mechanism of a hydroethanolic extract of B. monosperma flowers (BME) and more specifically of an enriched fraction in butrin and isobutrin (BI) was studied using cell culture of Normal Human Keratinocyte, cells involved in the skin inflammatory.Dried and crushed B. monosperma flowers were extracted with Ethanol/H2O (70/30 v/v). The butrin/isobutrin fraction was obtained by centrifugal Partition Chromatography (CPC). Experiments were conducted on UV-B treated normal human epidermis keratinocytes, cells involved in the skin inflammatory response. To evaluate extract anti-inflammatory activity, cytokines IL-1Î², IL-6, IL-8, prostaglandin E2 and metalloproteinases MMP-1, -2, -9 and -10 were measured in the cells supernatant.Our data clearly showed that hydroalcoholic B. monosperma flower extract was able to decrease the secretion of IL-1Î², IL-6 and IL-8 pro-inflammatory cytokines of -32, -33 and -18% respectively. Interestingly, Prostaglandin E2 production and the secretion of MMP-1, -2, -9 and -10 were also inhibited. Same results were observed in presence of enriched fraction in butrin and isobutrin and confirmed the participation of these molecules in the anti-inflammatory activity.These results explain the anti-inflammatory activity of B. monosperma and confirm the interest to use it in traditional Indian medicine. Moreover, its metalloproteinases inhibitory activities coupled with its anti-inflammatory action also give anti-aging property to this plant.Copyright Â© 2013 Elsevier Ireland Ltd. All rights reserved.
Spectroscopic and laser properties of SmÂ³âº ions doped lithium fluoroborate glasses for efficient visible lasers. - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
The Sm(3+)-doped lead barium zinc lithium fluoroborate (LBZLFB) glasses of composition 20PbO + 5BaO + 5ZnO + 10LiF + (60-x) B(2)O(3) + xSm(2)O(3), (where x=0.1, 0.5, 1.0, 1.5 and 2.0 mol%) have been prepared by conventional melt quenching technique and their structural and spectroscopic behavior were studied and reported. The amorphous nature of these glass samples was confirmed with XRD studies. The FT-IR and FT-Raman spectra reveal that, the glasses contain BO(3), BO(4), non-bridging oxygen and strong OH bonds. The bonding parameters and the oscillator strengths were determined from the absorption spectra. These parameters have been used to obtain the Judd-Ofelt intensity parameters. Using these intensity parameters various radiative and laser properties were predicted. The values of J-O intensity parameters suggested an increase in the degree of symmetry of the local ligand field at Sm(3+) sites. The decay rates for the (4)G(5/2) level of Sm(3+) ions have been measured and are found to be single exponential at lower concentrations (<1.0 mol%) and turn into non-exponential at higher concentrations (â‰¥1.0 mol%), due to energy transfer through cross-relaxation. From the emission characteristic parameters of (4)G(5/2) level, it is concluded that the LBZLFB glasses could be useful for photonic devices like visible lasers, fluorescent display devices and optical amplifiers, operated in the visible region.Copyright Â© 2012 Elsevier B.V. All rights reserved.
Is blood agar an alternative to sabouraud dextrose agar for the isolation of fungi in patients with mycotic keratitis. - International ophthalmology
To compare the blood agar (BA), sabouraud dextrose agar (SDA) and chocolate agar (CA) for the isolation of fungi in patients with mycotic keratitis. Corneal Scrapings of 229 patients with clinically diagnosed microbial keratitis were inoculated on BA, SDA, CA. The culture media were evaluated for the rate and time taken for the fungal growth. Seventy six of 229 patients had fungal keratitis. Fungus grew on BA in 60/76(78.9 %), on SDA in 76/76 (100 %), on CA in 40/76(52.6 %) patients. The fungi which grew on BA (60/76) also grown on SDA at the same time. The colony morphologies of different fungi were better on SDA than BA/CA. Among the different culture media, SDA is essential for the isolation fungi in patients with mycotic keratitis.
Crystal growth and characterization of Î³-glycine grown from potassium fluoride for photonic applications. - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Single crystals of Î³-glycine, an organic nonlinear optical material have been synthesized in the presence of potassium fluoride (KF) by slow evaporation technique at ambient temperature. The size of the grown crystal is up to the dimension of 12 mmÃ—10 mmÃ—8 mm. The Î³-phase was confirmed by single crystal X-ray diffraction, powder XRD and the FTIR analysis. Optical absorption spectrum reveals that the grown crystal has good optical transparency in the entire visible region with an energy band gap of 5.09 eV, which is an essential requirement for a nonlinear optical crystal. Thermal stability of the grown Î³-glycine crystal was determined using the thermo gravimetric and differential thermal analyses. The NLO activity of Î³-glycine was confirmed by the Kurtz powder technique using Nd:YAG laser and the grown crystal exhibits high relative conversion efficiency when compared to potassium dihydrogen phosphate (KDP).Copyright Â© 2011 Elsevier B.V. All rights reserved.
Posterior capsule dehiscence during phacoemulsification and manual extracapsular cataract extraction: comparison of outcomes. - Journal of cataract and refractive surgery
To compare the intraoperative behavior and postoperative outcomes of posterior capsule dehiscence during phacoemulsification and during manual extracapsular cataract extraction (ECCE).L.V. Prasad Eye Institute, Hyderabad, India.This study was a retrospective chart review of consecutive cases of posterior capsule dehiscence over 2 years in patients having phacoemulsification or manual ECCE. A chi-square test was used for statistical comparison of the results in the 2 groups.In the 127 eyes with posterior capsule dehiscence, the incidence of vitreous prolapse was comparable between the 2 groups (phacoemulsification, 61.6%; ECCE, 62.7%). In the phacoemulsification group, vitreous prolapse occurred more frequently if nuclear fragments were present at the time of posterior capsule dehiscence than if they were present during cortex removal (P =.05). Posterior dislocation of nuclear fragments occurred in 4 eyes in the phacoemulsification group. Increased anterior uveitis in the early postoperative period occurred more frequently in the ECCE group (P =.02). The visual outcomes were similar between the 2 groups.Even though differences existed between the intraoperative factors influencing the management of posterior capsule dehiscence during phacoemulsification and ECCE, the final anatomic and visual outcomes were comparable.
Allelic and locus heterogeneity in autosomal recessive gelatinous drop-like corneal dystrophy. - Human genetics
Gelatinous drop-like corneal dystrophy (GDLD) is a rare autosomal recessive disease characterized by the deposition of amyloid beneath the corneal epithelium and by severely impaired visual acuity leading to blindness. Although gelatinous corneal dystrophy has previously been mapped to chromosome 1p and seems to be associated with mutations in the M1S1 gene, molecular genetic studies have been limited to Japanese patients. To investigate the cause of GDLD in patients with diverse ethnic backgrounds, we performed linkage analyses in eight unrelated GDLD families from India, USA, Europe, and Tunisia. In seven of these families, the disease locus mapped to a 16-cM interval on the short arm of chromosome 1 between markers D1S519 and D1S2835, a region including the M1S1 gene. In addition, a 1.2-kb fragment containing the entire coding region of M1S1 gene was sequenced in affected individuals. Seven novel mutations (M1R, 8-bp ins., Q118 E, V194 E, C119 S, 870delC, and 1117delA) were identified in six families and two unrelated individuals. No sequence abnormalities were detected in a single family in which the GDLD locus was also excluded from the M1S1 region by linkage analysis. These findings demonstrate allelic and locus heterogeneity for GDLD.
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