Docality.com Logo
 
Dr. Melisa  Susanto  Dds image

Dr. Melisa Susanto Dds

16810 Meridian E Suite J104
Puyallup WA 98375
626 412-2669
Medical School: Other - Unknown
Accepts Medicare: No
Participates In eRX: No
Participates In PQRS: No
Participates In EHR: No
License #: 2901020349
NPI: 1396044517
Taxonomy Codes:
1223G0001X 1223P0221X

Request Appointment Information

Awards & Recognitions

About Us

Practice Philosophy

Conditions

Medical Malpractice Cases

None Found

Medical Board Sanctions

None Found

Referrals

None Found

Publications

Enhanced transthyretin tetramer stability following expression of an amyloid disease transsuppressor variant in mammalian cells. - The journal of gene medicine
The transthyretin (TTR) amyloidosis is an incurable fatal inherited disease that is characterized by progressive peripheral and autonomic neuropathy. It is caused by missense amyloidogenic mutations in the TTR gene that destabilize the native tetrameric state and lead to the cytotoxic misfolded monomeric state. One interesting variant (T119M) stabilizes heterotetramers with amyloidogenic TTR and, in the reported heterozygous individuals, protects the carriers from disease. In the present study, we characterize in vitro and in vivo the ectopic expression of the human T119M mutant, termed a transsuppressor for TTR amyloid disease.Lentiviral vectors encoding wild or mutant forms of human TTR were constructed and transduced to the human hepatocellular carcinoma cell line, HepG2, or mice. Heterooligomerization between T119M TTR and amyloidogenic variants was analysed by immunoprecipitation following western blotting.T119M TTR was stably expressed in transduced HepG2 cells and was secreted as an oligomer that can interact with its native partner, retinol-binding protein. Importantly, the T119M TTR formed secreted heterooligomers with amyloidogenic TTR variants, V30M, L55P and V122I, in HepG2 cells that were more stable than the homooligomers of the same amyloidogenic TTR variants. Human T119M TTR also formed heterooligomers with V30M TTR in transduced mice.The results obtained in the present study demonstrate the stabilization of heterotetramers by T119M TTR in human cells and suggest that gene transfer of T119M TTR may have potential as a gene therapy for TTR amyloidosis.Copyright (c) 2008 John Wiley & Sons, Ltd.

Map & Directions

16810 Meridian E Suite J104 Puyallup, WA 98375
View Directions In Google Maps

Nearby Doctors

16515 Meridian E Suite 104A
Puyallup, WA 98375
253 973-3030
16008 Meridian E
Puyallup, WA 98375
253 647-7645
16420 Meridian E #104
Puyallup, WA 98375
253 456-6669
16515 Meridian E Suite 104A
Puyallup, WA 98375
253 973-3030
15311 Canyon Rd E
Puyallup, WA 98375
253 312-2745
16420 Meridian E Suite 104
Puyallup, WA 98375
253 456-6669
16502 Meridian E Next To Walmart Vision Center
Puyallup, WA 98375
253 461-1760
16021 Meridian E
Puyallup, WA 98375
253 451-1600
16408 85Th Avenue Ct E
Puyallup, WA 98375
253 355-5422
18710 Meridian E Ste 116
Puyallup, WA 98375
253 759-9464