Dr. Shilpa  Madadi  Md image

Dr. Shilpa Madadi Md

1617 Hemphill St Department Of Internal Medicine
Fort Worth TX 76104
817 273-3941
Medical School: Other - Unknown
Accepts Medicare: No
Participates In eRX: No
Participates In PQRS: No
Participates In EHR: No
License #: N2179
NPI: 1356540330
Taxonomy Codes:

Request Appointment Information

Awards & Recognitions

About Us

Practice Philosophy


Medical Malpractice Cases

None Found

Medical Board Sanctions

None Found


None Found


Pharmacogenetic selection of transplanted human hepatocytes in immunocompetent rats. - Journal of digestive diseases
To introduce a genetic survival advantage for transplanted human hepatocytes over host cells in rats.Green fluorescent protein (GFP) was introduced into Huh-7 human hepatoma cells to create fluorescent GFP-Huh-7 cells. mRNA of CYP2E1, the enzyme that converts acetaminophen (APA) into hepatotoxic intermediates, was quantified by real-time polymerase chain reaction (PCR). The effects of APA on GFP-Huh-7 and control Huh-7 cells were determined in a cell culture. Immunological tolerance was induced by the injection of GFP-Huh-7 cells into fetal rats in utero. The GFP-Huh-7 cells were transplanted after birth of the rats into tolerant rats followed by APA treatment. Serum alanine aminotransferase (ALT) levels and liver histological data were obtained. GFP-Huh-7 cells were detected by quantitive PCR and microscopy.CYP2E1 mRNA levels in the GFP-Huh-7 cells were 2.7% of parental Huh-7 cells. In 1 mmol/L APA, parental Huh-7 cells decreased by 60% while GFP-Huh-7 cells increased to within 95% of untreated controls after 5 days. In rats in which GFP-Huh-7 cells were transplanted and treated with APA, serum ALT increased to a peak of 200 U/L on day 1 and returned to normal levels by day 3. Fluorescence microscopy of liver specimens from rats transplanted with GFP-Huh-7 cells showed substantial increases in GFP-Huh-7, but not Huh-7 cells by day 7 after APA treatment. Real-time PCR confirmed a 10-fold increase of GFP mRNA in APA-treated rats, but not in those without APA treatment.The difference in CYP2E1 gene expression between GFP-Huh-7 and rat hepatocytes provides a convenient means for the enrichment of transplanted human cells in rat liver.© 2012 The Authors. Journal of Digestive Diseases © 2012 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Wiley Publishing Asia Pty Ltd.

Map & Directions

1617 Hemphill St Department Of Internal Medicine Fort Worth, TX 76104
View Directions In Google Maps

Nearby Doctors

1301 Pennsylvania Ave Department Of Neonatology
Fort Worth, TX 76104
817 502-2892
800 8Th Ave Ste 116
Fort Worth, TX 76104
817 365-5633
900 8Th Ave
Fort Worth, TX 76104
817 475-5887
801 7Th Ave
Fort Worth, TX 76104
682 851-1475
1325 Pennsylvania Ave Ste 325
Ft Worth, TX 76104
817 879-9389
1500 S Main St
Fort Worth, TX 76104
817 213-3431
1001. 12Th Ave. 174
Fort Worth, TX 76104
817 480-0701
900 8Th Ave
Fort Worth, TX 76104
817 471-1140
1401 Pennsylvania Ave
Fort Worth, TX 76104
713 321-1100
900 8Th Ave
Fort Worth, TX 76104
817 775-5292