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Dr. Vani  Ray  Md image

Dr. Vani Ray Md

2000 E Layton Ave #250
St Francis WI 53235
414 827-7700
Medical School: Other - Unknown
Accepts Medicare: No
Participates In eRX: No
Participates In PQRS: No
Participates In EHR: No
License #: 36082
NPI: 1134236474
Taxonomy Codes:
2084P0800X

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Publications

DECT evaluation of noncalcified coronary artery plaque. - Medical physics
Composition of the coronary artery plaque is known to have critical role in heart attack. While calcified plaque can easily be diagnosed by conventional CT, it fails to distinguish between fibrous and lipid rich plaques. In the present paper, the authors discuss the experimental techniques and obtain a numerical algorithm by which the electron density (ρe) and the effective atomic number (Zeff) can be obtained from the dual energy computed tomography (DECT) data. The idea is to use this inversion method to characterize and distinguish between the lipid and fibrous coronary artery plaques.For the purpose of calibration of the CT machine, the authors prepare aqueous samples whose calculated values of (ρe, Zeff) lie in the range of (2.65 × 10(23) ≤ ρe ≤ 3.64 × 10(23)/cm(3)) and (6.80 ≤ Zeff ≤ 8.90). The authors fill the phantom with these known samples and experimentally determine HU(V1) and HU(V2), with V1,V2 = 100 and 140 kVp, for the same pixels and thus determine the coefficients of inversion that allow us to determine (ρe, Zeff) from the DECT data. The HU(100) and HU(140) for the coronary artery plaque are obtained by filling the channel of the coronary artery with a viscous solution of methyl cellulose in water, containing 2% contrast. These (ρe, Zeff) values of the coronary artery plaque are used for their characterization on the basis of theoretical models of atomic compositions of the plaque materials. These results are compared with histopathological report.The authors find that the calibration gives ρe with an accuracy of ±3.5% while Zeff is found within ±1% of the actual value, the confidence being 95%. The HU(100) and HU(140) are found to be considerably different for the same plaque at the same position and there is a linear trend between these two HU values. It is noted that pure lipid type plaques are practically nonexistent, and microcalcification, as observed in histopathology, has to be taken into account to explain the nature of the observed (ρe, Zeff) data. This also enables us to judge the composition of the plaque in terms of basic model which considers the plaque to be composed of fibres, lipids, and microcalcification.This simple and reliable method has the potential as an effective modality to investigate the composition of noncalcified coronary artery plaques and thus help in their characterization. In this inversion method, (ρe, Zeff) of the scanned sample can be found by eliminating the effects of the CT machine and also by ensuring that the determination of the two unknowns (ρe, Zeff) does not interfere with each other and the nature of the plaque can be identified in terms of a three component model.
Myoepithelial carcinoma of palate: case report. - Indian journal of pathology & microbiology
Myoepithelial carcinoma is an uncommon malignant salivary gland neoplasm with a predilection for parotid gland. However, its occurrence in minor salivary glands is considerably less with only 28 cases being reported in the literature. We report a case of myoepithelial carcinoma of palate in a 50-year-old woman. Computed tomography and magnetic resonance imaging revealed an extensive lesion in the palate. Microscopically, the tumor exhibited a wide spectrum of cytomorphologic features that overlap with other salivary gland tumors. Immunohistochemistry served as an adjunct in the diagnosis of the lesion. Clinical, radiographic, and histopathologic correlation along with immunohistochemical profile enabled to arrive at a diagnosis of myoepithelial carcinoma.
A novel surfactant-assisted ultrasound pretreatment of sugarcane tops for improved enzymatic release of sugars. - Bioresource technology
The aim of this study was to develop a novel surfactant-assisted ultrasound pretreatment of sugarcane tops as well as to optimize the effect of various operational parameters on pretreatment and hydrolysis. A novel surfactant-assisted ultrasound pretreatment was developed which could effectively remove hemicelluloses and lignin and improve the reducing sugar yield from sugarcane tops. Operational parameters for pretreatment and hydrolysis were studied and optimized. Under optimal hydrolysis conditions, 0.661 g of reducing sugar was produced per gram of pretreated biomass. The structural changes of native and pretreated biomass were investigated by Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared analysis (FTIR). The results indicate that surfactant-assisted ultrasound pretreated sugarcane tops can be used as a potential feed stock for bioethanol production.Copyright © 2012 Elsevier Ltd. All rights reserved.
An evaluation of chemical pretreatment methods for improving enzymatic saccharification of chili postharvest residue. - Applied biochemistry and biotechnology
Residue of chili plants left in the field after harvesting is a major lignocellulosic resource that is underexploited. India has over 0.6 million tons of this residue available as surplus annually which projects it as a potent feedstock for conversion to bioethanol. The cellulose, hemicellulose and lignin content of the chili residues are subject to variations with type of cultivar, geographical region and the season of cultivation, and the composition is critical in developing strategies for its conversion to bioalcohol(s). As with any lignocellulosic biomass, this feedstock needs pretreatment to make it more susceptible to hydrolysis by enzymes which is the most efficient method for generating sugars which can, then, be fermented to alcohol. Pretreatment of chili postharvest residue (CPHR) is, therefore, important though very little study has addressed this challenge. Similarly, enzymatic saccharification of pretreated chili biomass is another area which needs dedicated R&D because the combination of enzyme preparations and the conditions for saccharification are different in different biomass types. The present study was undertaken to develop an optimal process for pretreatment and enzymatic saccharification of CPHR that will yield high amount of free sugars. Dilute acid and alkali pretreatment of the biomass was studied at high temperatures (120-180 °C), with mixing (50-200 rpm) in a high pressure reactor. The holding time was adjusted between 15 and 60 min, and the resultant biomass was evaluated for its susceptibility to enzymatic hydrolysis. Similarly, the conditions for hydrolysis including biomass and enzyme loadings, mixing and incubation time were studied using a Taguchi method of experimentation and were optimized to obtain maximal yield of sugars. Efficiency of pretreatment was gauged by observing the changes in composition and the physicochemical properties of native and pretreated biomass which were analyzed by SEM and XRD analyses. The studies are expected to provide insights into the intricacies of biomass conversion leading to better processes that are simpler and more efficient.
Energy requirement for alkali assisted microwave and high pressure reactor pretreatments of cotton plant residue and its hydrolysis for fermentable sugar production for biofuel application. - Bioresource technology
In the present work, alkali assisted microwave pretreatment (AAMP) of cotton plant residue (CPR) with high pressure reactor pretreatment was compared. Further, modeling of AAMP was attempted. AAMP, followed by enzymatic saccharification was evaluated and the critical parameters were identified to be exposure time, particle size and enzyme loading. The levels of these parameters were optimized using response surface methodology (RSM) to enhance sugar yield. AAMP of CPR (1mm average size) for 6 min at 300 W yielded solid fractions that on hydrolysis resulted in maximum reducing sugar yield of 0.495 g/g. The energy required for AAMP at 300 W for 6 min was 108 kJ whereas high pressure pretreatment (180°C, 100 rpm for 45 min) requires 5 times more energy i.e., 540 kJ. Physiochemical characterization of native and pretreated feedstock revealed differences between high pressure pretreatment and AAMP.Copyright © 2012 Elsevier Ltd. All rights reserved.
Surfactant-assisted acid pretreatment of sugarcane tops for bioethanol production. - Applied biochemistry and biotechnology
Sugarcane tops is one of the largest biomass resources in India and in tropical countries such as Brazil in terms of surplus availability. Conversion of this feedstock to ethanol requires pretreatment to make it more accessible for the enzymes used in saccharification. Though several pretreatment regimens have been developed for addressing biomass recalcitrance, very few seem to be promising as an industrial process. A novel hybrid method involving use of mild acid and surfactant was developed which could effectively remove lignin and improve the sugar yield from sugar cane tops. Operational parameters that affect the pretreatment efficiency (measured as yield of sugars) were studied and optimized. Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored. The biomass pretreated with the optimized novel method could yield 0.798 g of reducing sugars per gram of pretreated biomass upon enzymatic hydrolysis.
Surfactant free rapid synthesis of hydroxyapatite nanorods by a microwave irradiation method for the treatment of bone infection. - Nanotechnology
Mesoporous nanocrystalline hydroxyapatite (nHAp) rods of size 40-75 nm long and 25 nm wide (resembling bone mineral) were synthesized under microwave irradiation without using any surfactants or modifiers. The surface area and average pore size of the nHAp were found to be 32 m(2) g(-1) and 4 nm, respectively. Rifampicin (RIF) and ciprofloxacin (CPF) loaded nHAp displayed an initial burst followed by controlled release (zero order kinetics). Combination of CPF and RIF loaded nHAp showed enhanced bacterial growth inhibition against Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis) and Escherichia coli (E. coli) compared to individual agent loaded nHAp and pure nHAp. In addition, decreased bacterial adhesion (90%) was observed on the surface of CPF plus RIF loaded nHAp. The biocompatibility test toward MG63 cells infected with micro-organisms showed better cell viability and alkaline phosphatase activity (ALP) for the combination of CPF and RIF loaded nHAp. The influence on cell viability of infected MG63 cells was attributed to the simultaneous and controlled release of CPF and RIF from nHAp, which prevented the emergence of subpopulations that were resistant to each other. Hence, apart from the issue of the rapid synthesis of nHAp without surfactants or modifiers, the simultaneous and controlled release of dual drugs from nHAp would be a simple, non-toxic and cost-effective method to treat bone infections.
Tubular aggregate myopathy: a phenotypic spectrum and morphological study. - Neurology India
Tubular aggregates (TAs) are inclusions described in skeletal muscle in a variety of disorders. In a retrospective analysis, TAs were found in 18 (0.24%) cases and involved a spectrum of clinical phenotypes. Ultrastructurally, four distinct types of aggregates were noted. There was no correlation between the clinical phenotypes, duration of illness and types of TAs.
Hydrothermal synthesis of porous triphasic hydroxyapatite/(alpha and beta) tricalcium phosphate. - Journal of materials science. Materials in medicine
A novel, porous triphasic calcium phosphate composed of nonresorbable hydroxyapatite (HAp) and resorbable tricalcium phosphate (alpha- and beta-TCP) has been synthesized hydrothermally at a relatively low temperature. The calcium phosphate precursor for hydrothermal treatment was prepared by gel method in the presence of ascorbic acid. XRD, FT-IR, Raman analyses confirmed the presence of HAp/TCP. The surface area and average pore size of the samples were found to be 28 m2/g and 20 nm, respectively. The samples were found to be bioactive in simulated body fluid (SBF).
Eventration of the diaphragm and associations. - Indian journal of pediatrics
Eventration of the diaphragm, most often an isolated entity and detected incidentally, has been known to be associated with several genetic syndromes. Authors report their experience of seeing diaphragmatic eventration in association with Poland syndrome and wandering spleen syndrome and briefly discuss the literature.

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